Diabetes ameliorative effect of solvent extract of Bauhinia acuminata bark in streptozotocin induced diabetic male albino rats
Sajani Singharoy1, Shibani Das1, Dibya Pal1, and Debidas Ghosh1,2∗
1Department of Bio-Medical Laboratory Science and Management, Vidyasagar University, West Midnapore, West Bengal, India
2Department of Medical Laboratory Technology, Debra Thana Sahid Kshudiram Smriti Mahavidyalaya, Debra, West Midnapore, West Bengal, India
Abstract: Diabetes is a lifestyle illness that is on the rise and is linked to long-term metabolic problems. This study uses Wistar strain albino male rats as experimental diabetic model animals to assess the anti-diabetic and anti-oxidative effects of hydro-methanol (3:2), hydro-ethanol (3:2), and aqueous extracts of Bauhinia acuminata bark. Rats were divided into control, diabetic, and extract-treated diabetic groups. Following diabetes induction by streptozotocin injection, rats were administered the respective extracts for 28 days at a dose of 20 mg/0.5 ml distilled water per 100 g body weight. Fasting blood glucose levels were monitored. Muscular glycogen levels, toxicity indicators (GOT, GPT), oxidative stress markers (catalase, SOD, TBARS), carbohydrate metabolic enzyme activities (hexokinase, glucose-6-phosphatase), and histological features of the pancreas were also evaluated. All assessed parameters showed significant improvement in extract-treated diabetic groups compared to vehicle-treated diabetic controls. Among the extracts, the hydro-ethanol (3:2) extract exhibited the most pronounced restoration of glycemic balance, oxidative stress markers, and enzymatic activities, along with evidence of beta-cell regeneration. These findings suggest that the hydro-ethanol extract of Bauhinia acuminata bark possesses potent anti-diabetic and anti-oxidative properties, offering promising therapeutic potential for the management of diabetes and its associated metabolic disturbances.
Key Words: Anti-diabetic; Anti-oxidative; Bauhinia acuminata; Histological view of pancreas